Abstrakt

BDNF Promotes EGF-Induced Proliferation and Migration of Human Fetal Neural Stem/Progenitor Cells via the PI3K

Yi Li Wu

Neurogenesis is a complex process, which contributes to the capability of the adult brain to serve typically and acclimatize to conditions. Epidermal growth factor (EGF) is known to play an important part in neurogenesis; still, the beginning medium is still unclear. Then, we hypothecated that brain- deduced neurotrophic factor (BDNF) can enhance the effect of EGF on neurogenesis. Using in vitro cell culture of aborted mortal fetal brain apkins, we delved proliferation and migration of neural stem/ ancestor cells (NSPCs) after treatment with EGF and different attention of BDNF. EGF stimulated proliferation and migration of NSPCs, and this effect was significantly enhanced byco-incubation with BDNF. In the NSPCs treated with 50 ng/ mL BDNF, BrdU objectification was significantly increased (from7.91 to17.07), as compared with that in the control. Also, the number of migrating cells was at least2-fold advanced than that in the control