Abstrakt

Involvement of CUL4A in Regulation of Multidrug Resistance to P-gp Substrate Drugs in Breast Cancer Cells

M Harpaz

Adr cells independently. Using rear recap polymerase chain responses and western spots, we set up that overexpression of CUL4A in MCF7 and MDA- MB- 468 cells up- regulated MDR1/ Pgp expression on both the recap and protein situations, which conferred multidrug resistance to P-gpsubstrate medicines, as determined by 3,5-dimethylthiazol-2-yl) platitude( MTT) assays. On the contrary hand, silencing CUL4A in MCF7/ Adr and MDA- MB- 468/ Adr cells led to the other effect. also, ERK1/ 2 in CUL4A- overexpressing cells was largely actuated and after treatment with PD98059, an ERK1/ 2-specific asset, CUL4A- convinced expression of MDR1/ Pgp was dropped significantly. Incipiently, immunohistochemistry in melanoma napkins showed that P-gpexpression had a positive correlation with the expression of CUL4A and ERK1/2. Therefore, these results inferred that CUL4A and ERK1/ 2 shared inmate- drug resistances in melanoma through regulation of MDR1/ Pgp expression.